UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM N-Q QUARTERLY SCHEDULE OF PORTFOLIO HOLDINGS OF REGISTERED MANAGEMENT INVESTMENT COMPANIES Investment Company Act file number 811-8568 John Hancock Bank & Thrift Opportunity Fund (Exact name of registrant as specified in charter) 601 Congress Street, Boston, Massachusetts 02210 (Address of principal executive offices) (Zip code) Alfred E. Ouellette, Senior Attorney & Assistant Secretary 601 Congress Street Boston, Massachusetts 02210 (Name and address of agent for service) Registrant's telephone number, including area code: 617-663-4324 Date of fiscal year end: October 31 Date of reporting period: July 31, 2005 ITEM 1. SCHEDULE OF INVESTMENTS JOHN HANCOCK Bank and Thrift Opportunity Fund 7.31.2005 Quarterly Portfolio Holdings [A 2" x 1" John Hancock (Signature)/John Hancock Funds logo in lower, center middle of page. A tag line below reads "JOHN HANCOCK FUNDS."] John Hancock Bank and Thrift Opportunity Fund Securities owned by the Fund on July 31, 2005 (unaudited) Interest Maturity Credit Par value Issuer, description rate (%) date rating ($000) Value --------------------------------------------------------------------------------------------------------------------------------- Bonds 0.09% $912,065 (Cost $770,000) Regional Banks 0.09% 912,065 CSBI Capital Trust I, Gtd Sub Cap Inc Ser A (B)(G) 11.750 06-06-2027 B- 770 912,065 Issuer Shares Value --------------------------------------------------------------------------------------------------------------------------------- Common stocks 90.45% $899,419,861 (Cost $385,265,281) Asset Management & Custody Banks 4.37% 43,490,113 Affiliated Managers Group, Inc. (MA) (I)(L) 200,750 14,313,475 Bank of New York Co., Inc. (The) (NY) 313,000 9,634,140 Federated Investors, Inc. (Class B) (PA) 30,000 958,200 Legg Mason, Inc. (MD) 10,250 1,047,038 Mellon Financial Corp. (PA) 330,000 10,051,800 Northern Trust Corp. (IL) 70,000 3,556,000 State Street Corp. (MA) 79,000 3,929,460 Consumer Finance 0.25% 2,516,000 MBNA Corp. (DE) 100,000 2,516,000 Diversified Banks 13.63% 135,518,754 Bank of America Corp. (NC) 498,170 21,720,212 Comerica, Inc. (MI) 447,400 27,336,140 Toronto-Dominion Bank (The) (Canada) 140,499 6,422,209 U.S. Bancorp. (MN) 878,541 26,408,942 Wachovia Corp. (NC) 535,071 26,956,877 Wells Fargo & Co. (CA) 434,861 26,674,374 Other Diversified Financial Services 3.89% 38,700,678 Citigroup, Inc. (NY) 505,925 22,007,737 JPMorgan Chase & Co. (NY) 475,041 16,692,941 Regional Banks 61.84% 614,886,499 ABC Bancorp. (GA) 78,480 1,569,600 Alabama National Bancorp. (AL) 142,500 9,882,375 Amegy Bancorp., Inc. (TX) 520,660 11,860,635 AmericanWest Bancorp. (WA) (I) 357,921 8,196,391 AmSouth Bancorp. (AL) 95,879 2,675,983 BB&T Corp. (NC) 344,846 14,421,460 Benjamin Franklin Bancorp., Inc. (MA) 15,000 208,050 Beverly National Corp. (MA) 47,500 1,227,875 BOK Financial Corp. (OK) 117,208 5,705,685 Camden National Corp. (ME) 140,000 5,363,400 Capital City Bank Group, Inc. (FL) 74,543 2,769,272 Cardinal Financial Corp. (VA) (I) 86,200 894,756 Cascade Bancorp. (OR) 194,272 4,464,371 Chittenden Corp. (VT) 175,245 5,132,926 City Holding Co. (WV) 41,600 1,595,360 City National Corp. (CA) 222,927 16,289,276 CoBiz, Inc. (CO) 11,650 213,195 Colonial BancGroup, Inc. (The) (AL) 585,200 13,617,604 Columbia Bancorp. (MD) 90,000 3,752,100 Columbia Bancorp. (OR) 13,250 276,263 Columbia Banking System, Inc. (WA) 29,645 820,574 Commercial Bankshares, Inc. (FL) 63,702 2,504,126 Community Bancorp. (NV) (I) 10,790 356,178 Community Banks, Inc. (PA) (L) 105,055 2,913,175 Compass Bancshares, Inc. (AL) 563,357 27,159,441 Cullen/Frost Bankers, Inc. (TX) 20,000 1,002,000 Dearborn Bancorp., Inc. (MI) (I) 41,310 1,074,060 Desert Community Bank (CA) 137,500 3,678,139 DNB Financial Corp. (PA) 69,121 1,745,305 East West Bancorp., Inc. (CA) 500,000 17,250,000 EuroBancshares, Inc. (Puerto Rico) (I) 87,830 1,469,396 F.N.B. Corp. (PA) 90,049 1,778,468 Fifth Third Bancorp. (OH) (L) 510,040 21,982,724 Financial Institutions, Inc. (NY) 73,000 1,406,710 First Charter Corp. (NC) 52,200 1,291,950 First Horizon National Corp. (TN) 156,650 6,389,753 First Midwest Bancorp., Inc. (IL) 49,000 1,828,680 First National Lincoln Corp. (ME) 146,499 2,636,982 First Regional Bancorp. (CA) (I) 150,000 12,213,000 First State Bancorp. (NM) 130,000 2,886,000 FirstMerit Corp. (OH) 2,950 83,456 Fulton Financial Corp. (PA) 133,503 2,411,064 Glacier Bancorp., Inc. (MT) 367,788 10,504,025 Harleysville National Corp. (PA) 137,776 3,093,071 Hibernia Corp. (Class A) (LA) 234,700 7,937,554 Independent Bank Corp. (MI) 308,105 9,514,282 International Bancshares Corp. (TX) 200,337 6,056,188 KeyCorp (OH) (L) 320,000 10,956,800 Lakeland Financial Corp. (IN) 7,000 300,650 M&T Bank Corp. (NY) (L) 238,657 25,896,671 Marshall & Ilsley Corp. (WI) (L) 584,795 26,853,786 MB Financial, Inc. (IL) 123,950 5,156,320 Mercantile Bankshares Corp. (MD) 209,500 11,656,580 Merrill Merchants Bankshares, Inc. (ME) 75,897 1,783,579 Mid-State Bancshares (CA) 50,000 1,544,000 National City Corp. (DE) 550,687 20,325,857 North Fork Bancorp., Inc. (NY) 887,092 24,297,450 Northrim Bancorp., Inc. (AK) 70,053 1,693,181 Oriental Financial Group, Inc. (Puerto Rico) 20,234 322,732 Pacific Capital Bancorp. (CA) 364,354 12,468,194 Placer Sierra Bancshares (CA) 14,550 433,445 PNC Financial Services Group, Inc. (PA) 418,500 22,942,170 Prosperity Bancshares, Inc. (TX) 125,000 3,850,000 Provident Bankshares Corp. (MD) 165,058 5,611,972 Regions Financial Corp. (AL) (L) 127,390 4,285,400 Republic Bancorp., Inc. (MI) 3,300 48,840 S&T Bancorp., Inc. (PA) 154,700 6,152,419 Sandy Spring Bancorp., Inc. (MD) 9,500 328,035 Security Bank Corp. (GA) 70,000 1,661,100 Sky Financial Group, Inc. (OH) 234,850 6,688,528 SNB Bancshares, Inc. (TX) (I) 94,340 1,084,910 Southwest Bancorp., Inc. (OK) 41,000 940,950 Summit Bancshares, Inc. (TX) 279,000 5,102,910 SunTrust Banks, Inc. (GA) (L) 346,826 25,221,187 Synovus Financial Corp. (GA) 450,000 13,306,500 Taylor Capital Group, Inc. (IL) 213,200 7,809,516 TCF Financial Corp. (MN) 428,016 11,757,600 TD Banknorth, Inc. (ME) 334,517 9,961,916 Texas United Bancshares, Inc. (TX) 47,100 906,675 TriCo Bancshares (CA) 53,000 1,286,840 UCBH Holdings, Inc. (CA) (L) 400,000 7,308,000 Umpqua Holdings Corp. (OR) 177,901 4,435,072 Univest Corp. (PA) 205,218 6,386,384 Valley National Bancorp. (NJ) 157,386 3,706,440 Vineyard National Bancorp., Co. (CA) 283,970 9,481,758 Virginia Commerce Bancorp., Inc. (VA) (I) 16,671 436,113 Virginia Financial Group, Inc. (VA) 22,400 819,616 West Coast Bancorp. (OR) 67,583 1,770,675 Western Alliance Bancorp. (NV) (I) 20,650 638,085 Whitney Holding Corp. (LA) 159,750 5,290,920 Wilmington Trust Corp. (DE) 350,000 13,128,500 Yardville National Bancorp. (NJ) 97,400 3,526,854 Zions Bancorp. (UT) (L) 324,825 23,218,491 Specialized Finance 0.23% 2,233,484 CIT Group, Inc. (NJ) 50,600 2,233,484 Thrifts & Mortgage Finance 6.24% 62,074,333 Astoria Financial Corp. (NY) (L) 107,865 3,013,748 Commercial Capital Bancorp., Inc. (CA) 450,000 8,928,000 Countrywide Financial Corp. (CA) 291,500 10,494,000 Freddie Mac (VA) 140,000 8,859,200 Hingham Institute for Savings (MA) 80,000 3,356,800 Hudson City Bancorp., Inc. (NJ) 383,810 4,540,472 LSB Corp. (MA) 65,000 1,114,750 New York Community Bancorp., Inc. (NY) 40,000 734,400 NewAlliance Bancshares, Inc. (CT) 12,581 181,795 PennFed Financial Services, Inc. (NJ) 313,600 5,948,992 Sovereign Bancorp., Inc. (PA) 30,000 719,700 Washington Mutual, Inc. (WA) 254,312 10,803,174 Webster Financial Corp. (CT) 70,110 3,379,302 Interest Par value Issuer, description, maturity date rate (%) ($000) Value --------------------------------------------------------------------------------------------------------------------------------- Short-term investments 9.46% $94,055,939 (Cost $94,055,939) Certificates of Deposit 0.01% 81,467 Deposits in mutual banks 81,467 Joint Repurchase Agreement 2.22% 22,034,000 Investment in a joint repurchase agreement transaction with Bank of America Corp. - Dated 07-29-05 due 08-01-05 (secured by U.S. Treasury STRIP'S due 11-15-10 thru 08-15-27) 3.300 22,034 22,034,000 Shares Cash Equivalents 7.23% 71,940,472 AIM Cash Investment Trust (T) 71,940,472 71,940,472 Total investments 100.00% $994,387,865 John Hancock Bank and Thrift Opportunity Fund Footnotes to Schedule of Investments July 31, 2005 (unaudited) (B) This security is fair valued in good faith under procedures established by the Board of Trustees. (G) Security rated internally by John Hancock Advisers, LLC. (I) Non-income-producing security. (L) All or a portion of this security is on loan as of July 31, 2005. (T) Represents investment of securities lending collateral. Parenthetical disclosure of a foreign country in the security description represents country of a foreign issuer. The percentage shown for each investment category is the total value of that category as a percentage of the total investments of the Fund. The cost of investments owned on July 31, 2005, including short-term investments, was $480,091,220. Gross unrealized appreciation and depreciation of investments aggregated $515,065,986 and $769,341, respectively, resulting in net unrealized appreciation of $514,296,645. For more information Trustees Ronald R. Dion, Chairman James R. Boyle+ James F. Carlin Richard P. Chapman, Jr.* William H. Cunningham Charles L. Ladner* Dr. John A. Moore* Patti McGill Peterson* Steven R. Pruchansky +Non-Independent Trustee *Members of the Audit Committee Officers Keith F. Hartstein President and Chief Executive Officer William H. King Vice President and Treasurer Francis V. Knox, Jr. Vice President and Chief Compliance Officer John G. Vrysen Executive Vice President and Chief Financial Officer Investment Adviser John Hancock Advisers, LLC 601 Congress Street Boston, Massachusetts 02210-2805 Custodian The Bank of New York One Wall Street New York, New York 10286 Transfer Agent and Dividend Disburser Mellon Investor Services 85 Challenger Road Overpeck Centre Ridgefield Park, New Jersey 07660 Legal Counsel Wilmer Cutler Pickering Hale and Dorr LLP 60 State Street Boston, Massachusetts 02109-1803 Stock Symbol Listed New York Stock Exchange: BTO How to contact us Internet www.jhfunds.com Mail Regular mail: Mellon Investor Services 85 Challenger Road Overpeck Centre Ridgefield Park, NJ 07660 Phone Customer service representatives 1-800-852-0218 Portfolio commentary 1-800-344-7054 24-hour automated information 1-800-843-0090 TDD line 1-800-231-5469 This report is for the information of the shareholders of John Hancock Bank and Thrift Opportunity Fund. P90Q3 7/05 9/05 ITEM 2. CONTROLS AND PROCEDURES. (a) Based upon their evaluation of the registrant's disclosure controls and procedures as conducted within 90 days of the filing date of this Form N-Q, the registrant's principal executive officer and principal accounting officer have concluded that those disclosure controls and procedures provide reasonable assurance that the material information required to be disclosed by the registrant on this report is recorded, processed, summarized and reported within the time periods specified in the Securities and Exchange Commission's rules and forms. (b) There were no changes in the registrant's internal control over financial reporting that occurred during the registrant's last fiscal quarter that has materially affected, or is reasonably likely to materially affect, the registrant's internal control over financial reporting. ITEM 3. EXHIBITS. Separate certifications for the registrant's principal executive officer and principal accounting officer, as required by Rule 30a-2(a) under the Investment Company Act of 1940, are attached. SIGNATURES Pursuant to the requirements of the Securities Exchange Act of 1934 and the Investment Company Act of 1940, the registrant has duly caused this report to be signed on its behalf by the undersigned, thereunto duly authorized. John Hancock Bank and Thrift Opportunity Fund By: /s/ Keith F. Hartstein ------------------------------------- Keith F. Hartstein President and Chief Executive Officer Date: September 30, 2005 Pursuant to the requirements of the Securities Exchange Act of 1934 and the Investment Company Act of 1940, this report has been signed below by the following persons on behalf of the registrant and in the capacities and on the dates indicated. By: /s/ Keith F. Hartstein ------------------------------------- Keith F. Hartstein President and Chief Executive Officer Date: September 30, 2005 By: /s/ John G. Vrysen ------------------------------------- John G. Vrysen Executive Vice President and Chief Financial Officer Date: September 30, 2005